Journal: Biology
Article Title: Force-Dependent Presence of Senescent Cells Expressing Vascular Endothelial Growth Factor During Orthodontic Tooth Movement
doi: 10.3390/biology15020187
Figure Lengend Snippet: VEGF-expressing senescent ECs. ( A ) Representative p16, CD31, and VEGF triple immunofluorescence staining images. p16/CD31/VEGF staining without DAPI ( left ) and merged images with DAPI ( right ) are shown. ( B ) Quantitative analysis of p16 + CD31 + VEGF + area in PDL. ( C ) Schematic illustration of the quantitative analysis for p16, CD31, and VEGF in PDL. ( D ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + CD31 + area. ( E ) Quantitative analysis of p16 + CD31 + VEGF + area/p16 + VEGF + area. Scale bars: 100 µm (low magnification), 10 µm (high magnification). Data are mean ± SD (n = 4). ns: not significant, ** p < 0.01; **** p < 0.0001; Kruskal–Wallis with Dunn’s post hoc test for ( B ), and one-way ANOVA with Tukey’s test for ( D , E ). VEGF: vascular endothelial growth factor, PDL: periodontal ligament, AB: alveolar bone.
Article Snippet: CD31 , Endothelial cell marker , Bioss Antibodies (Shanghai, China) , bs-0195R , BF647 , 1:100.
Techniques: Expressing, Immunofluorescence, Staining